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restriction mapping中文是什么意思

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用"restriction mapping"造句"restriction mapping"怎么读"restriction mapping" in a sentence

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  • 限制制图

例句与用法

  • The restriction map of phzl318 carrying the entire add gene cluster from s . avermitilis nrrl8165 was made . its two subclones phz2104 and phz2105 were introduced into s . lividans mutant zx1
    制作了携带完整add基因簇的phz1318的限制内切酶图谱,根据酶谱进行亚克隆得到phz2104和phz2105 。
  • By comparing restriction maps of plasmids possessed in these clones , 5 clones ( clones 1 , 4 , 5 , 6 , and 8 ) were found to contain the same chitinase gene , while the other three clones ( clones 2 , 3 and 9 ) contain different chitinase genes one another
    经底物反应和限制性内切酶图谱分析,确定其中的clone - 1 , 4 , 5 , 6 , 8含有相同的几丁质酶基因;而clone - 2 , 3 , 5 , 9四株重组菌则含有不同的几丁质酶基因。
  • The restriction map of the plasmid pbl29 was tentatively constructed according as the molecular weights of fragments of plasmid pbl29 digested with different enzymes . analysing the sequence of the plasmid pbl29 tested , we found that the mol ecular weight of the plasmid pbl29 is 371 lbp , that it s many unique restriction endonucleases , km resistance genes and abundant a / t base pairs of replicating site are on the plasmid pbl29
    同时根据限制性酶切各片段的分子量作出了质粒pbl29的内切酶图谱。并对质粒pbl29进行测序和分析,证明了质粒pbl29大小为3711bp ,具有大量的单酶切位点、编码卡那霉素抗性基因以及富含a t序列的复制起点。
  • Then , the plasmid was transformed into jm109 . the full length pstvd cdna recombinant plasmid was further identified by restriction mapping analysis and the nucleotide sequence of cdna cloned in pmd 18 - t vector was analyzed with ab1 377 dna sequence . test showed that the cdna of this chinese pstvd isolate was the same as pstvd - kf - 6 mild " type " isolate which originated from naturally infected potatoes in field of cornell university potato breeding program
    利用rt - pgr技术,设计一对引物,对田间采集的经鉴定含pstvd的阳性样品进行全序列扩增,并将所得产物纯化回收,连接到pmd18t - vector中,并转化至大肠杆菌jm109中,挑选白斑进行双酶切( saci / ecorv )鉴定证明插入片段为359bp大小,进行序列测定,所得克隆基因为pstvd全序列负链,大小为359bp 。
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